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Image Search Results
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Article Title: A-485 alleviates fibrosis and apoptosis in kidney by disrupting tandem activation of acetylation and phosphorylation on STAT3.
doi: 10.1016/j.biopha.2025.118217
Figure Lengend Snippet: Fig. 7. Effects of A-485 on acetylation and phosphorylation of STAT3 in UUO kidney. (A) The representative western blot images and quantification of p-STAT3 (Y705), p-STAT3(S727), ac-STAT3(K685) and total STAT3 in Sham and UUO mice (n = 6). (B) The representative western blot images and quantification of p-STAT3 (Y705), p-STAT3 (S727), ac-STAT3 (K685) and total STAT3 in UUO mice with or without A-485 treatment (n = 6). (C-D) The quantification of p-STAT3(Y705) and ac-STAT3 (K685) expression in UUO mice detected by immunohistochemical staining (n = 6, scale bar = 50μm). (E) The representative western blot images and quantification of p300 and CBP in Sham and UUO mice (n = 6). (F) The representative western blot images and quantification of p300 and CBP in UUO mice with or without A-485 treatment (n = 6). (G) The p300 catalytic activity in mice kidney (n = 6). Values are expressed as means ± SEM. *p < 0.05, * *p < 0.01. Sham, sham operation; UUO, unilateral ureteral obstruction. CBP, CREB binding protein.
Article Snippet: The antibodies used in the present study are listed as below: antibodies against Bcl-2 (ab196495), Collagen I (ab34710); antibodies against STAT3 (10253–2-AP), Bax (50599–2-Ig), α-SMA (14395–1-AP), Fibronectin (15613–1-AP), E-Cadherin (20874–1-AP) and GAPDH (10494–1-AP) (Proteintech, Chicago, IL, USA);
Techniques: Phospho-proteomics, Western Blot, Expressing, Immunohistochemical staining, Staining, Activity Assay, Binding Assay
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Article Title: A-485 alleviates fibrosis and apoptosis in kidney by disrupting tandem activation of acetylation and phosphorylation on STAT3.
doi: 10.1016/j.biopha.2025.118217
Figure Lengend Snippet: Fig. 8. Effects of A-485 on acetylation and phosphorylation of STAT3 in TGF-β1-induced HK-2 cells. (A) The representative western blot images and quantification of p-STAT3(Y705), p-STAT3(S727), ac-STAT3 (K685) and STAT3 in HK-2 cells treated with different A-485 concentrations (n = 3). (B) The immunofluorescence staining of p-STAT3(Y705) and ac-STAT3 (K685) in HK-2 cells (scale bar = 50μm). (C) The representative western blot images and quantification of p300 and CBP in HK-2 cells treated with different A-485 concentrations (n = 3). (D) The p300 catalytic activity in HK-2 cells (n = 5). Values are expressed as means ± SEM. *p < 0.05, * *p < 0.01. CBP, CREB binding protein.
Article Snippet: The antibodies used in the present study are listed as below: antibodies against Bcl-2 (ab196495), Collagen I (ab34710); antibodies against STAT3 (10253–2-AP), Bax (50599–2-Ig), α-SMA (14395–1-AP), Fibronectin (15613–1-AP), E-Cadherin (20874–1-AP) and GAPDH (10494–1-AP) (Proteintech, Chicago, IL, USA);
Techniques: Phospho-proteomics, Western Blot, Immunofluorescence, Staining, Activity Assay, Binding Assay
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Article Title: A-485 alleviates fibrosis and apoptosis in kidney by disrupting tandem activation of acetylation and phosphorylation on STAT3.
doi: 10.1016/j.biopha.2025.118217
Figure Lengend Snippet: Fig. 9. The relationship between phosphorylation and acetylation of STAT3 in TGF-β1 stimulated HK-2 cells. (A) The representative western blot images and quantification of p-STAT3 (Y705), ac-STAT3 (K685) and STAT3 in HK-2 cells stimulated with different TGF-β1 concentrations (n = 3). (B) The representative western blot images and quantification of p-STAT3 (Y705), ac-STAT3 (K685) and STAT3 in HK-2 cells with the transfection of the plasmids of STAT3 WT, K685R, K685Q (n = 3). (C) The representative western blot images and quantification of p-STAT3 (Y705), ac-STAT3 (K685) and STAT3 in HK-2 cells with the transfection of the plasmids of STAT3 WT, Y705F, Y705D (n = 3). (D) The immunofluorescence staining of p-STAT3(Y705), ac-STAT3 (K685), α-SMA in HK-2 cells (scale bar = 50μm). Values are expressed as means ± SEM. * *p < 0.01. KO, knockout; WT, wild type.
Article Snippet: The antibodies used in the present study are listed as below: antibodies against Bcl-2 (ab196495), Collagen I (ab34710); antibodies against STAT3 (10253–2-AP), Bax (50599–2-Ig), α-SMA (14395–1-AP), Fibronectin (15613–1-AP), E-Cadherin (20874–1-AP) and GAPDH (10494–1-AP) (Proteintech, Chicago, IL, USA);
Techniques: Phospho-proteomics, Western Blot, Transfection, Immunofluorescence, Staining, Knock-Out